[Mir wurde bereits geholfen... Danke]

[phip]

Hallo... Ich bin in Englisch ne Niete und daher brauche ich mal kurz eine Bestätigung, ob ich folgendes so schreiben kann... Ich will mich ja nicht schon mit einer kurzen Anfangszusammenfassung blamieren....
Ist nichts dramatisch wichtiges, aber trotzdem wär fehlerfreies Englisch angebracht...

______________

The main objective of this work was to examine, whether the expression of miR-301a in different LPS activated leucocytes is dependent on the expression of miR-155. To answer this question two different methods were performed: On the one hand a classical Northern Blot approach and on the other hand qRT-PCRs. Both methods could not confirm the assumption of miR-301a is regulated by the expression of miR-155.
The second focus of this work was on finding new, biologically important targets of miR-155 und miR-301a. To solve this problem first of all different databases were searched for potential new Targets. After that die 3’UTR-sequences were cloned into a dual-luciferase-reporter plasmid. The following dual-luciferase assays coluld not demonstrate that one of the examined 3’UTRs is negatively regulated by miR-155.
____________________

(P.s.: Ich muss endlich ins Ausland... Mit so einer Englisch-Unsicherheit wird das nix in Zukunft... )

[Chipper]

Hi
Okay, das Fachchinesisch kann ich nicht beurteilen , aber ein paar andere Verbesserungen kann ich beitragen:
Komma nach examine raus (1.Zeile)
nach Doppelpunkt klein weiter (4.Zeile)
Der letzte Satz des 1. Abschnitts müßte wohl lauten: ... assumption that miR.... (zumindest so wie ich den Sinn der Ausführung verstehe)
targets klein 3. Zeile Abs 2

Ich hoffe, dir ist damit geholfen.

Zitat von phip

Hallo... Ich bin in Englisch ne Niete und daher brauche ich mal kurz eine Bestätigung, ob ich folgendes so schreiben kann... Ich will mich ja nicht schon mit einer kurzen Anfangszusammenfassung blamieren....
Ist nichts dramatisch wichtiges, aber trotzdem wär fehlerfreies Englisch angebraucht...

______________

The main objective of this work was to examine, whether the expression of miR-301a in different LPS activated leucocytes is dependent on the expression of miR-155. To answer this question two different methods were performed: On the one hand a classical Northern Blot approach and on the other hand qRT-PCRs. Both methods could not confirm the assumption of miR-301a is regulated by the expression of miR-155.
The second focus of this work was on finding new, biologically important targets of miR-155 und miR-301a. To solve this problem first of all different databases were searched for potential new Targets. After that die 3’UTR-sequences were cloned into a dual-luciferase-reporter plasmid. The following dual-luciferase assays coluld not demonstrate that one of the examined 3’UTRs is negatively regulated by miR-155.
____________________

(P.s.: Ich muss endlich ins Ausland... Mit so einer Englisch-Unsicherheit wird das nix in Zukunft... )

[fAiThLezZ]

Zitat von phip

The main objective of this work was to examine, whether the expression of miR-301a in different LPS activated leucocytes is dependent(würde hier depending nehmen) on the expression of miR-155. To answer this question two different methods were performed: On the one hand a classical Northern Blot approach and on the other hand qRT-PCRs. Both methods could not confirm the assumption ofthat miR-301a is regulated by the expression of miR-155.
The second focus of this work was on to find new, biologically important targets of miR-155 und miR-301a. (To solve this problem) First of all different databases were searched for potential new targets. After that die(meintest du "the"?) 3’UTR-Sequences were cloned(cloned?heißt doch dann wohl ein identisches oder hat sich etwas verändert??Wolltest du transferieren damit ausdrücken?) into a dual-luciferase-reporter plasmid. The following dual-luciferase assays coluldcould? not demonstrate that one of the examined 3’UTRs is negatively regulated by miR-155.

Habs nur kurz überflogen, könnten noch Verschlimmbesserungen dabei sein. Ich hoffe du kannst die inhaltliche Unsicherheit verzeihen :-D

[birnchen]

Zitat von phip

The main objective of this work was to examine [ ] whether the expression of miR-301a in different LPS-activated leucocytes depends on the expression of miR-155. To answer this question, two different methods - a classical Northern Blot approach and qRT-PCRs - have been investigated. Both methods could not confirm the assumption that miR-301a is regulated by the expression of miR-155.
The second focus of this work was on finding new, biologically important targets of miR-155 and miR-301a. To solve this problem, different databases were searched for potentially new targets initially. Afterwards, the 3’UTR [ ] sequences were cloned into a dual-luciferase [ ] reporter plasmid. The following dual-luciferase assays could not point out that one of the examined 3’UTRs is negatively regulated by miR-155.

Hab dir mal nen paar Vorschläge gemacht.

[phip]

Danke Leute... Ich kann mich nur bedanken...